Journal: Molecular Therapy Oncology

Article Title: Induced pluripotent stem cell-derived dendritic cells potentiate the efficacy of interleukin-12-expressing oncolytic HSV-1

doi: 10.1016/j.omton.2026.201252

Figure Lengend Snippet: Combination of T-mfIL12 with immature BMDCs or iPSDCs in the subcutaneous MB49 tumor model (A) Combination of T-mfIL12 with immature BMDCs in the subcutaneous MB49 tumor model. A marked inhibition of tumor growth was observed in the T-mfIL12+BMDC group. Significant tumor growth inhibitions compared to the mock group were also seen in the T-01+BMDC and T-mfIL12 monotherapy groups. n = 6 mice per group. Error bars represent standard error of the mean (SEM). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. (B) Combination of T-mfIL12 with immature iPSDCs in the subcutaneous MB49 tumor model. A significant inhibition of tumor growth was observed in the T-mfIL12+iPSDC group, which was comparable to the inhibition seen in the T-mfIL12+BMDC group. n = 6 mice per group. Error bars represent SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: The murine bladder cancer cell line MB49, originally induced by 7,12-dimethylbenz[a]anthracene, was kindly provided by Dr. Timothy L. Ratliff (Purdue University, USA; RRID: CVCL_7076).

Techniques: Inhibition

Journal: Molecular Therapy Oncology

Article Title: Induced pluripotent stem cell-derived dendritic cells potentiate the efficacy of interleukin-12-expressing oncolytic HSV-1

doi: 10.1016/j.omton.2026.201252

Figure Lengend Snippet: Immunological assessments of the combination of T-mfIL12 and immature BMDCs (A) Immunohistochemical analysis of representative MB49 tumors from the indicated treatment groups. Increased intratumoral infiltration of CD8 + lymphocytes was most prominent in the T-mfIL12+BMDC group, followed by the T-01+BMDC group. In both groups, elevated levels of CD4 + lymphocyte infiltration were also observed in the same tumor sections. Other groups, except for the mock group, showed moderate levels of CD4 + infiltration. Scale bars: 100 μm. (B) ELISpot assay for IFN-γ expression revealed the highest number of IFN-γ-producing splenocytes in the T-mfIL12+BMDC group, followed by the T-01+BMDC group. Monotherapies with BMDC, T-01, or T-mfIL12, as well as the mock group, did not result in significant increases. n = 3 per group; all samples were analyzed in triplicate. Error bars represent standard deviation (SD). ∗ p < 0.05, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: The murine bladder cancer cell line MB49, originally induced by 7,12-dimethylbenz[a]anthracene, was kindly provided by Dr. Timothy L. Ratliff (Purdue University, USA; RRID: CVCL_7076).

Techniques: Immunohistochemical staining, Enzyme-linked Immunospot, Expressing, Standard Deviation

Journal: Molecular Therapy Oncology

Article Title: Induced pluripotent stem cell-derived dendritic cells potentiate the efficacy of interleukin-12-expressing oncolytic HSV-1

doi: 10.1016/j.omton.2026.201252

Figure Lengend Snippet: Immunological assessments of the combination of T-mfIL12 and immature iPSDCs (A) Immunohistochemical analysis of representative MB49 tumors from the indicated treatment groups. Increased intratumoral infiltration of CD8 + lymphocytes was observed in both the T-mfIL12+BMDC and T-mfIL12+iPSDC groups. In both groups, CD4 + lymphocyte infiltration was also more prominently observed in the same tumor sections. Other treatment groups, excluding the mock group, exhibited moderate levels of CD4 + T cell infiltration. Scale bars: 100 μm. (B) ELISpot assay showed a high and comparable increase in IFN-γ-producing splenocytes in both the T-mfIL12+BMDC and T-mfIL12+iPSDC groups. n = 3 mice per group; all samples were analyzed in triplicate. Error bars represent standard deviation (SD). ∗∗∗∗ p < 0.0001; NS, not significant.

Article Snippet: The murine bladder cancer cell line MB49, originally induced by 7,12-dimethylbenz[a]anthracene, was kindly provided by Dr. Timothy L. Ratliff (Purdue University, USA; RRID: CVCL_7076).

Techniques: Immunohistochemical staining, Enzyme-linked Immunospot, Standard Deviation

Journal: Molecular Therapy Oncology

Article Title: Induced pluripotent stem cell-derived dendritic cells potentiate the efficacy of interleukin-12-expressing oncolytic HSV-1

doi: 10.1016/j.omton.2026.201252

Figure Lengend Snippet: Immunohistochemical analysis of DC surface markers in MB49 tumors Representative tumor sections were stained for CD11c (a marker of DC presence) and CD86 (a marker of DC maturation). Tumors from the BMDC and iPSDC monotherapy groups were CD11c-positive but CD86-negative, indicating the presence of immature DCs. In contrast, tumors from the T-mfIL12+BMDC and T-mfIL12+iPSDC groups were positive for both CD11c and CD86, suggesting the presence of mature DCs. Tumors from the mock and T-mfIL12 monotherapy groups were negative for both markers. These findings suggest that the co-administration of T-mfIL12 may promote DC maturation in the tumor microenvironment. Scale bars: 100 μm.

Article Snippet: The murine bladder cancer cell line MB49, originally induced by 7,12-dimethylbenz[a]anthracene, was kindly provided by Dr. Timothy L. Ratliff (Purdue University, USA; RRID: CVCL_7076).

Techniques: Immunohistochemical staining, Staining, Marker

Journal: International Journal of Molecular Sciences

Article Title: Growth Hormone Signaling in Bladder Cancer: Transcriptomic Profiling of Patient Samples and In Vitro Evidence of Therapy Resistance via ABC Transporters and EMT Activation

doi: 10.3390/ijms26157113

Figure Lengend Snippet: Human and mouse UC cells express GHR. ( a ) The human UC cell line UM-UC-3 expresses GHR mRNA, confirmed by qPCR. This cell line also exhibits relatively high expression of other genes involved in GH signaling, including GH1 , IGF1R , and IGFBP3. ( b ) The murine UC cell line MB49 expresses Ghr mRNA, confirmed by qPCR. This cell line also exhibits relatively high levels of Igf1r and Insr . ( c , d ) High expression of GHR at the protein level was also confirmed by western blot in human and mouse UC cells.

Article Snippet: UM-UC-3 (human UC; purchased from ATCC #CRL-1749, Manassas, VA, USA) and MB49 (C57BL/6-background murine UC; purchased from Sigma-Aldrich #SCC148, St. Louis, MO, USA) were maintained in EMEM (ThermoFisher #11095098, Waltham, MA, USA) and DMEM growth media (ThermoFisher #11995040), respectively, supplemented with 10% fetal bovine serum (FBS; ThermoFisher #10082147) and 1% penicillin-streptomycin (ThermoFisher #15140122), referred to hereafter as complete growth medium, at 37 °C and 5% CO 2 .

Techniques: Expressing, Western Blot